hek-293ft cell line  (Thermo Fisher)

Journal: BMC Complementary Medicine and Therapies

Article Title: Formulation of 1% α-mangostin in orabase gel induces apoptosis in oral squamous cell carcinoma

doi: 10.1186/s12906-024-04450-0

Figure Lengend Snippet: Cytotoxicity of formulation of 1% α -MG in orabase gel in 293FT cell line

Article Snippet: Human embryonic kidney (HEK) 293FT cell line was purchased from Invitrogen company (Carlsbad, CA, USA).

Techniques: Formulation, Concentration Assay

Journal: BMC Complementary Medicine and Therapies

Article Title: Formulation of 1% α-mangostin in orabase gel induces apoptosis in oral squamous cell carcinoma

doi: 10.1186/s12906-024-04450-0

Figure Lengend Snippet: Effect of the formulation of 1% α-MG in orabase gel on HPV-16 pseudovirus infection in 293FT cell. HPV-16 pseudovirus at multiplicity of infection (MOI) at 0.05 was performed to infect 293FT cells. For pre-attachment step ( A ), the formulation of 1% α-MG in orabase gel (Formulation 2) at CC 20 and CC 50 was mixed to pseudovirus and incubated at 37 °C for 1 h. Subsequently, the mixture was incubated with 293FT cells for 4 h and then removed and replaced with complete medium incubated for 48 h. For adsorption step ( B ), the 293FT cells were adsorbed with pseudovirus at 20 °C for 2 h. The gel was added to the cells after removal of the pseudovirus and then incubated for 48 h. Heparin (400 µM) was used as a positive control. Total cells and green fluorescent cells were counted in the same area by hemocytometer. The number of green fluorescent cells was relative to total cells and compared to untreated cells. The different percentage of inhibition was statistically assessed by an unpaired t test. The significant value was indicated as ** ( P < 0.01) and *** ( P < 0.001), respectively

Article Snippet: Human embryonic kidney (HEK) 293FT cell line was purchased from Invitrogen company (Carlsbad, CA, USA).

Techniques: Formulation, Infection, Incubation, Adsorption, Positive Control, Inhibition